GUIDE How to test THC / CBD?
How to test THC / CBD and other cannabinoids: if you intend to use a psychoactive substance or hemp first you need a test kit to check it’s purity and potency. Cannabis products such as weed, hash, oil, cakes can not be identified by appearance. Check your sample with a single or multi-use Cannabinoids test kit.
Cannabis tester
Recommended CBD / THC testing kits
Recommended multi-use CBD / THC Reagent Test Kit includes reagents Cannabis (4-AP), Zimmermann and a magnifying glass. Reagents help rule out presence of synthethic cannabinoids and estimate CBD and THC potency. To discover full cannabinoids profile and estimate THC and CBD potency % pair reagents with the TLC Cannabinoids Test Kit. To detect fentanyl use the fent strips.
STEP ONE How to detect synthetic cannabinoids, CBD and THC?
How to use reagent tests?
TL;DR: Below is an image with a quick cheat sheet how to use the CBD / THC Test Kit and further down you can check out more detailed guide how to test cannabis products. Use the CBD / THC reagent test chart to see how to use Cannabis reagent and Zimmermann. Check the expected results.
CBD / THC test results chart:
TEST | CBD | THC |
CANNABIS (4-AP) | Purple | Blue |
ZIMMERMANN | Purple | No instant vivid color change |
What are CBD / THC test kit reagents used for?
SAMPLE | RECOMMENDED CBD / THC REAGENT TESTS |
FLOWERS (MARIJUANA, WEED) EXTRACTS (HASH, OIL), FOOD |
|
Reagent tests indicate presence of a substance, therefore can either help confirm presence of the expected compound or help rule out certain cuts.
- Cannabis (4-AP) – main test for THC / CBD, helps rule out synthetic cannabinoids
- Zimmermann / Beam – main test for cathinones and benzodiazepines in geneal, detects also CBD
- Single use cannabis test kit includes Cannabis reagent
- Multi use CBD / THC reagent test kit includes reagents Cannabis and Zimmermann
Cannabis reagent (4-AP) indicates if sample contains mostly THC (blue color change), mostly CBD (pink or purple color change) or no cannabinoids at all. Confirming THC dominant sample helps rule out presence of synthethic cannabinoids (source: saferparty.ch research partnership). Zimmermann reagent helps detect CBD and compare CBD potency between samples.
CBD / THC test kit results
How to test CBD potency?
How to test THC potency?
Questions?
STEP TWO How to test CBD and THC potency %?
To discover all cannabinoids and estimate quantity pair reagent tests with a TLC (Thin Layer Chromatography) Purity Test Kit. TLC purity tests alone don’t identify substances and are best paired with at least 1-2 reagent tests. Thin Layer Chromatography is one of classic substance analysis methods. It works by separating different compounds depending on their solubility in chosen TLC testing liquid (eluent). To precisly estimate THC and CBD potency pair a reagent test kit with the TLC Cannabinois Test Kit.
How to test quality of CBD oil, THC oil, flowers and other cannabis products?
Qualitative TLC:
TLC test works by separating substances present in a sample, each detected spot indicates a different substance.
- Kit comes with a calibrated ruler which indicates where to look for specific cannabinoids such as CBD, THC, THCV, CBC, CBG, CBN.
- You can use an already confirmed sample for reference – if both compounds show up at the same height on the TLC “card” that indicates they can be the same substance..
TLC Cannabis Potency Test results
TLC THC Potency Test scale
How to test quality of THC oil, CBD oil, flowers and other cannabis products?
Semi-quantitative TLC:
TLC helps estimate quantity (potency). The bigger the size of the detected spot, the bigger the amount of measured substance. Note that different substances need different calibrated rulers and often different testing liquids (eluents).
- Use a calibrated potency % ruler to estimate result with +-1% accuracy
- One spot indicates a pure substance. If your sample fully dissolved in testing liquid and only one spot was detected on TLC then your substance is pure. Trace amounts of impurities might not be detected, do not rely on TLC for fentanyl testing – get also fentanyl test strips
How to use TLC cannabis potency test?
Questions?
How to test weed for fentanyl?
Use fentanyl test strips (a fentanyl test kit)
METHOD 1
Add small sample to a plastic baggie. If testing a pill crush it in the baggy and dump out the powder. Then add 10 drops of water into the baggie and mix well. Dip the test strip no higher than the thick line. Wait 15 seconds. Put the strip away and check in 5 minutes.
METHOD 2
Mix 100 mg of sample in 200 ml of water. Dip the test strip no higher than the thick line. Wait 15 seconds. Put the strip away and check in 5 minutes.
COMPARE RESULTS
1 line = fentanyl, 2 lines = no fentanyl. Faint lines also count. Ignore fentanyl tester results after 5 minutes.
To detect all ingredients and estimate their amount pair reagents with a TLC purity test kit.
A positive or negative test result does not indicate if a substance is safe. No substance is 100% safe.